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Cannabis Business Insights | Friday, May 12, 2023
Analysts have developed a whole raft of different analytical testing techniques.
FREMONT, CA: Over the past decade, analysts have developed a whole raft of different analytical testing techniques – many adapted from techniques common in the food and drink industries – to find new and improved ways of protecting consumer health from dangerous contaminants.
1. Cannabis potency testing
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Cannabis potency testing determines how much CBD and THC are present in a product.
Several labs prefer utilizing GC-FID(gas chromatography and a flame ionization detector) for fast and simple analysis. Liquid samples are vaporized and then sent through a gas chromatograph. The respective compounds in the sample dissolve into prudent parts according to their chemical closeness with the column. The divided compounds are then fed into a flame that ionizes them before passing across a detector. By gauging the current produced as the ions pass the detector's electrode, it is feasible to correctly measure all the organic chemical species.
While GC-FID is a quick solution for potency study, full cannabinoid profiling needs a more direct technique. Thus, liquid chromatography is chosen. It enables analysts to gauge the number of cannabidiol acids in a sample, not only how much CBDA or THCA is transformed to CBD and THC in the certain heating requirements of a gas chromatograph.
High-performance liquid chromatography accompanying a diode array detector (HPLC-DAD) and liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS) is the most generally chosen systems often employed later being a more high-resolution solution for scientific research laboratories.
With betterments in instrumentation and separation technology, HPLC-DAD has achieved favor for cannabinoid analysis for different reasons, comprising short run times and minimum limits of quantification for every analyte.
In HPLC-DAD, the analytes are isolated through a liquid chromatography column and examined based on the diode array detector's ultraviolet and visible (UV-vis) absorption spectra. LC-TOF-MS uses high-resolution accurate mass (HRAM) spectrometry to explain matrix effects and other chemical hindrances. Yet, the sophistication of this technique implies it is predominantly employed in the research sector instead of in the wider testing industry.
2. Cannabis pesticide testing
The target lists for which pesticides must be screened vary greatly between states and countries. And with no integrated set of testing standards or standard analytes, it is common that the techniques utilized by testing laboratories also differ significantly from lab to lab.
As a common rule, the testing workflow incorporates an extraction step designed to capture any pesticides on the material & a cleanup step to eradicate any pigments or interferences that may impact the testing outcomes. The following sample is then analyzed using LC/MS/MS or GC/MS/MS(liquid or gas chromatography with tandem mass spectrometry).
The analysis was conventionally performed on a liquid chromatograph with a tandem triple quadrupole mass spectrometer(LC/MS/MS). A fistful of compounds is more compliant with a gas chromatography-mass spectrometry(GC-MS) analysis. So there are quite some groups that are using a GC-MS to study those compounds as well.
3. Heavy metals testing
Cannabis plants be prone to "pull" heavy metals out of their encircling soil and absorb them into the plant as they cultivate.
Testing cannabis for heavy metal pollution is usually done through one of three atomic spectrometry-based methods:
• Atomic absorption (AA)
• Inductively coupled plasma optical emission spectroscopy (ICP-OES)
• Inductively coupled plasma mass spectrometry (ICP-MS)
AA is a simple technique that employs the principle of the Beer-Lambert Law to gauge the concentration of specific analytes present by studying the wavelengths of light absorbed by unrestricted gaseous atoms of the sample. The minimum cost of this analysis has made it the most common analysis method of this type. Yet, its slow sample throughput can be a hindrance.
Instead, labs with a larger budget opt for ICP-MS analysis, which ionizes the elements in a sample through inductively associated plasma. The ions can then be fed into the mass spectrometer and examined as per their respective mass-to-charge ratios. While it is a more pricey technique, the fast runtimes, high throughput, and superior sensitivity of ICP-MS are a strong pull for greater laboratory operations.
ICP-OES is a middle ground between the two other atomic spectrometry techniques, more inexpensive than ICP-MS and more effective than AA. It employs ICP to excite the elements present in the sample so that they start to articulate photons of a distinct wavelength. By gauging the brightness and wavelength of this light that is emitted, it is likely to recognize and quantify any heavy metals present. While it cannot contact the same parts per trillion levels of sharpness that ICP-MS can, provided the right sample preparation technique, it can still notice actually into the low parts per billion range.
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